Brilliant Green Bile Broth 2% was first described by Durham and Schoenlein1 to selectively recover organisms of the coli-aerogenes group. The concentration of the components of the medium is optimized so that anaerobic lactose fermenters are inhibited without the coliforms being affected.

• Easy-to-read: coliform bacteria are easy to recognize by rapid production of gas through lactose fermentation.
• Selects for coliform organisms: bile salts and brilliant green in the medium inhibit nontarget Gram-positive and Gram-negative organisms. The medium is further optimized to prevent the growth of anaerobic lactose fermenters without coliforms being affected.